Rat PCX (Podocalyxin) ELISA Kit | ELK4896

Rat PCX (Podocalyxin) ELISA Kit | ELK4896 | Gentaur UK, US & Europe Distribution

Alternative Names: Gp200; PCLP; PC; PODXL; Podocalyxin Like Protein 1; GCTM-2 antigen

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Hematology;Kidney biomarker

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin (PCX) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Podocalyxin (PCX) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocalyxin (PCX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Podocalyxin (PCX) in the samples is then determined by comparing the OD of the samples to the standard curve.