Rat Period circadian protein homolog 2 (PER2) ELISA Kit | KTE100484

Rat Period circadian protein homolog 2 (PER2) ELISA Kit | KTE100484 | Gentaur UK, US & Europe Distribution

Application: This Rat Period circadian protein homolog 2 (PER2) ELISA Kit employs a two-site sandwich ELISA to quantitate PER2 in samples. An antibody specific for PER2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPER2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PER2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PER2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Period circadian protein homolog 2 microplate
• Rat Period circadian protein homolog 2 standard
• Rat Period circadian protein homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Period circadian protein homolog 2 (PER2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PER2. No significant cross-reactivity or interference between Rat PER2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PER2 is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. A new genetic test from a cheek swab can use Per2 expression levels to tell whether a person is an early morning person or a "night owl". It has also been shown that Per2 and Bmal1 work in opposition to each other.

Alternative Names: PER2; FASPS; KIAA0347; period 2; period circadian protein 2

Search name: PER2; FASPS; KIAA0347; period 2; period circadian protein 2

Tag: PER2