Rat PFKL (Phosphofructokinase, Liver) ELISA Kit | ELK6626

Rat PFKL (Phosphofructokinase, Liver) ELISA Kit | ELK6626 | Gentaur UK, US & Europe Distribution

Alternative Names: PFK-B; 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; Phosphohexokinase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.67 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphofructokinase, Liver (PFKL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphofructokinase, Liver (PFKL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphofructokinase, Liver (PFKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphofructokinase, Liver (PFKL) in the samples is then determined by comparing the OD of the samples to the standard curve.