Rat PICP (Procollagen I C-Terminal Propeptide) ELISA Kit | ELK2376

Rat PICP (Procollagen I C-Terminal Propeptide) ELISA Kit | ELK2376 | Gentaur UK, US & Europe Distribution

Alternative Names: P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.54 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Hepatology;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I C-Terminal Propeptide (PICP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen I C-Terminal Propeptide (PICP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I C-Terminal Propeptide (PICP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I C-Terminal Propeptide (PICP) in the samples is then determined by comparing the OD of the samples to the standard curve.