Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit | KTE100891

Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit | KTE100891 | Gentaur UK, US & Europe Distribution

Application: This Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 microplate
• Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 standard
• Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HCN3. No significant cross-reactivity or interference between Rat HCN3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.

Alternative Names: HCN3; KIAA1535; MGC131493; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Search name: HCN3; KIAA1535; MGC131493; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Tag: HCN3