Rat PROCR (Protein C Receptor, Endothelial) ELISA Kit | ELK2477

Rat PROCR (Protein C Receptor, Endothelial) ELISA Kit | ELK2477 | Gentaur UK, US & Europe Distribution

Alternative Names: CD201; CCCA ; CCD41; BA42O4.2; EPCR; Endothelial Protein C Receptor; Cell Cycle, Centrosome-Associated Protein; Activated Protein C Receptor

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C Receptor, Endothelial (PROCR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein C Receptor, Endothelial (PROCR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C Receptor, Endothelial (PROCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C Receptor, Endothelial (PROCR) in the samples is then determined by comparing the OD of the samples to the standard curve.