Rat Prostaglandin reductase 2 (PTGR2) ELISA Kit | KTE100392

Rat Prostaglandin reductase 2 (PTGR2) ELISA Kit | KTE100392 | Gentaur UK, US & Europe Distribution

Application: This Rat Prostaglandin reductase 2 (PTGR2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGR2 in samples. An antibody specific for PTGR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Prostaglandin reductase 2 microplate
• Rat Prostaglandin reductase 2 standard
• Rat Prostaglandin reductase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Prostaglandin reductase 2 (PTGR2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTGR2. No significant cross-reactivity or interference between Rat PTGR2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PTGR2 is an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13, 14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. ZADH1 has a domain characteristic of the zinc-containing ADH family, a proline-rich motif, and a putative NADP (+) -binding domain. RT-PCR detected ubiquitous expression of the shorter ZADH1 transcript (ZADH1b), with highest expression in kidney, liver, pancreas, prostate, and heart, followed by lung, skeletal muscle, testis, small intestine, and brain. Expression was low in all other tissues examined.

Alternative Names: PTGR2; DKFZp686P10120; FLJ39091; FLJ99229; PGR2; ZADH1; 15-oxoprostaglandin-delta13-reductase; zinc binding alcohol dehydrogenase; domain containing 1

Search name: PTGR2; DKFZp686P10120; FLJ39091; FLJ99229; PGR2; ZADH1; 15-oxoprostaglandin-delta13-reductase; zinc binding alcohol dehydrogenase; domain containing 1

Tag: PTGR2