Rat PRSS1 (Protease, Serine 1) ELISA Kit | ELK2337

Rat PRSS1 (Protease, Serine 1) ELISA Kit | ELK2337 | Gentaur UK, US & Europe Distribution

Alternative Names: TRP1; TRY1; TRY4; TRYP1; Trypsin 1; Cationic Trypsinogen; Beta-trypsin; Serine protease 1; Trypsin I

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.245 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 1 (PRSS1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protease, Serine 1 (PRSS1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 1 (PRSS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease, Serine 1 (PRSS1) in the samples is then determined by comparing the OD of the samples to the standard curve.