Rat REG1a (Regenerating Islet Derived Protein 1 Alpha) ELISA Kit | ELK6018

Rat REG1a (Regenerating Islet Derived Protein 1 Alpha) ELISA Kit | ELK6018 | Gentaur UK, US & Europe Distribution

Alternative Names: REG; PSP; ICRF; PSPS1; PTP; Islet cells regeneration factor; Islet of Langerhans regenerating protein; Lithostathine 1 Alpha; Pancreatic Stone Protein; Pancreatic Thread Protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.288 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Endocrinology;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Alpha (REG1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 1 Alpha (REG1a) in the samples is then determined by comparing the OD of the samples to the standard curve.