Description
Rat SCD (Stearoyl Coenzyme A Desaturase) ELISA Kit | ELK6966 | Gentaur UK, US & Europe Distribution
Alternative Names: FADS5; SCDOS; Fatty Acid desaturase 5; Delta-9-Desaturase; Stearoyl-CoA Desaturase Opposite Strand
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.6 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stearoyl Coenzyme A Desaturase (SCD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Stearoyl Coenzyme A Desaturase (SCD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stearoyl Coenzyme A Desaturase (SCD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stearoyl Coenzyme A Desaturase (SCD) in the samples is then determined by comparing the OD of the samples to the standard curve.