Rat SGLT2 (Sodium/Glucose Cotransporter 2) ELISA Kit | ELK6918

Rat SGLT2 (Sodium/Glucose Cotransporter 2) ELISA Kit | ELK6918 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC5A2; Solute Carrier Family 5 Member 2; Low affinity sodium-glucose cotransporter; Na (+) /glucose cotransporter 2

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.28 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Glucose Cotransporter 2 (SGLT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Glucose Cotransporter 2 (SGLT2) in the samples is then determined by comparing the OD of the samples to the standard curve.