Rat SPA2 (Surfactant Associated Protein A2) ELISA Kit | ELK5499

Rat SPA2 (Surfactant Associated Protein A2) ELISA Kit | ELK5499 | Gentaur UK, US & Europe Distribution

Alternative Names: SFTPA2B; COLEC5; SP2A; SPA1 beta; SP-A2; SPAII; Collectin-5; Pulmonary Surfactant Protein A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein A2 (SPA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Surfactant Associated Protein A2 (SPA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein A2 (SPA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein A2 (SPA2) in the samples is then determined by comparing the OD of the samples to the standard curve.