Description
Rat SPON2 (Spondin 2) ELISA Kit | ELK7130 | Gentaur UK, US & Europe Distribution
Alternative Names: DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.068 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Spondin 2 (SPON2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the OD of the samples to the standard curve.