Rat Thy1 (Thy1 Cell Surface Antigen) ELISA Kit | ELK6396

Rat Thy1 (Thy1 Cell Surface Antigen) ELISA Kit | ELK6396 | Gentaur UK, US & Europe Distribution

Alternative Names: CD90; CDw90; THY mocyte differentiation antigen 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Hematology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1 Cell Surface Antigen (Thy1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thy1 Cell Surface Antigen (Thy1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thy1 Cell Surface Antigen (Thy1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thy1 Cell Surface Antigen (Thy1) in the samples is then determined by comparing the OD of the samples to the standard curve.