Rat TNFSF4 (Tumor Necrosis Factor Ligand Superfamily, Member 4) ELISA Kit | ELK9207

Rat TNFSF4 (Tumor Necrosis Factor Ligand Superfamily, Member 4) ELISA Kit | ELK9207 | Gentaur UK, US & Europe Distribution

Alternative Names: CD252; TXGP1; OX40L; Gp34; OX40-L; Tax-Transcriptionally Activated Glycoprotein 1, 34kD; Glycoprotein Gp34; OX40 ligand

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.3 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) in the samples is then determined by comparing the OD of the samples to the standard curve.