Rat TP53 (Tumor Protein p53) ELISA Kit | ELK1509

Rat TP53 (Tumor Protein p53) ELISA Kit | ELK1509 | Gentaur UK, US & Europe Distribution

Alternative Names: p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Species: Rat

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein p53 (TP53) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Protein p53 (TP53) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein p53 (TP53), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein p53 (TP53) in the samples is then determined by comparing the OD of the samples to the standard curve.