Rat TPH1 (Tryptophan Hydroxylase 1) ELISA Kit | ELK6096

Rat TPH1 (Tryptophan Hydroxylase 1) ELISA Kit | ELK6096 | Gentaur UK, US & Europe Distribution

Alternative Names: TPH; TPRH; TRPH; Tryptophan 5-Monooxygenase

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.24 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan Hydroxylase 1 (TPH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tryptophan Hydroxylase 1 (TPH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan Hydroxylase 1 (TPH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan Hydroxylase 1 (TPH1) in the samples is then determined by comparing the OD of the samples to the standard curve.