Rat WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit | E-EL-R2578

Rat WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit | E-EL-R2578 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: A0A0G2K6Q8

Target Name: WNT10B

Target Synonym: N/A

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat WNT10B. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat WNT10B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat WNT10B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat WNT10B. You can calculate the concentration of Rat WNT10B in the samples by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Rat WNT10B in samples. No significant cross-reactivity or interference between Rat WNT10B and analogues was observed.

Research Areas: Cancer, Metabolism, Neuroscience, Stem cells

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.