RBR1 | Retinoblastoma related protein | AS11 1627

RBR1 | Retinoblastoma related protein | AS11 1627 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format: Liquid

Tested Application: Chromatin IP (ChIP), Immunoprecipitation (IP), Western blot (WB)

Related Products: Collection of antibodies for plant developmental biologyPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 2µl (IP), 1 : 2000 (WB)

Molecular weight: 112 kDa

Confirmed Reactivity: Arabidopsis thaliana, Medicago sativa

Predicted Reactivity: Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea mays Species of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii

Additional Information: This antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane) .For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.

Background: The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR

Reconstitution: N/A

Storage: Store at 4°C. Upon arrival Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G12280

Category: Cell cycle

Research Area: Cell Biology