RE | N-terminal arginylation | AS15 3050

RE | N-terminal arginylation | AS15 3050 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Dot blot (Dot)

Related Products: AS15 3050A | Anti-RD | N-terminal arginylation, rabbit antibodies collection of antibodies to proteins involved in transcriptioncollection of antibodies to proteins involved in translationPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (Dot)

Molecular weight: N/A

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins

Background: Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.

Reconstitution: For reconstitution add 50 µl of sterile water to each tube

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Translation

Research Area: Epigenetics