REC8 Antibody (Center) [APR03837G]

REC8 Antibody (Center) [APR03837G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This REC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-222 amino acids from the Central region of human REC8.

Cellular Localisation: Nucleus {ECO:0000250|UniProtKB:Q6AYJ4}. Chromosome {ECO:0000250|UniProtKB:Q6AYJ4}. Chromosome, centromere {ECO:0000250|UniProtKB:Q6AYJ4}. Note=In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. Localised to centromeres and spindle poles in endopolyploid tumor cells {ECO:0000250|UniProtKB:Q6AYJ4}

Molecular Weight: 62614

Clone: Polyclonal

Gene Name: REC8

Gene ID: 9985

Function: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity) .

Summary: Tissue Location: Expressed in testis and thymus. Expressed in the B- cell lines WI-L2-NS and Namalwa (at protein level)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1