RECK Antibody (Center) [APR32676G]

RECK Antibody (Center) [APR32676G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RECK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-456 amino acids from the Central region of human RECK.

Cellular Localisation: Cell membrane; Lipid-anchor, GPI-anchor

Molecular Weight: 106457

Clone: Polyclonal

Gene Name: RECK {ECO:0000303|PubMed:9789069, ECO:0000312|HGNC:HGNC:11345}

Gene ID: 8434

Function: Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314) . Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity) . Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed:30026314) . ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314) . Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:9789069, PubMed:18194466) . Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:9789069) .

Summary: Tissue Location: Expressed in various tissues and untransformed cells (PubMed:9789069) . It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:2000

Synonyms: Reversion-inducing cysteine-rich protein with Kazal motifs, hRECK, Suppressor of tumorigenicity 15 protein, RECK, ST15