Recombinant hSUMO1-AMC | 6412

hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

6412 | Recombinant hSUMO1-AMC DataSheet

Biomolecule/Target: N/A

Synonyms: Recombinant hSUMO1-AMC

Alternates names: N/A

Taglines: Pure and sensitive AMC tagged fluorogenic substrate for deSUMOylating enzymes.

NCBI Gene ID #: 6288

NCBI Gene Symbol: SAA1

Gene Source: Human

Accession #: P0DJI8

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: RP-HPLC

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 11.142 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in 0.1% acetic acid to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A