Description
Recombinant Human FGF-2 (147) | GFH28-50
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release.Description
Fibroblast Growth Factor 2 (FGF-2) is expressed by endothelial cells and is a mediator of angiogenesis. FGF-2 also has cardioprotective functions during heart injury. The application of FGF-2 is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length FGF-2 N-terminus results in a truncated FGF-2 147 amino acids protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-2 within the cell, but do not affect the biological activity of FGF-2. Therefore, there are no detectable differences in biological activity between the full length FGF-2 154 amino acids and the truncated FGF-2 147 amino acids recombinant proteins.Activity Assay
Human FGF-2 (147) is fully biologically active when compared to standard. The activity is determined by the dose-dependent induced proliferation of NR6R 3T3 cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.AA Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKSAdditional Information
Source: |
E. coli |
Purity: |
≥95% determined by reducing and non-reducing SDS-PAGE |
Endotoxin Level: |
≤1.00 EU/μg as measured by kinetic LAL |
Formulation: |
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5 |
Stability and Storage: |
12 months from date of receipt when stored at -20°C to -80°C as supplied. |
Packing: |
50 ug |