Recombinant NKX2.2 Antibody [NX2/1422R] | 34-086

Recombinant NKX2.2 Antibody [NX2/1422R] | 34-086 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Research Area: Stem Cell

Tested Application: Flow, IHC-P, IF

Application: Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE) : 1-2 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein A affinity chromatography

Clonality: Recombinant Monoclonal

Clone: NX2/1422R

Isotype: IgG, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.