Recombinant SARS-CoV-2 Papain-like Protease | P1551

SARS-CoV is an enveloped, single and positive-stranded RNA virus. Replication of the genomic RNA of SARS-CoV is mediated by replicase polyproteins that are processed by two viral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro). The chymotrypsin-like 3CLpro processes the replicase polyprotein at 11 sites, including cleaving itself from the polyprotein to generate a ∼25-kDa protease product. Papain-like proteases have been shown to process the amino-terminal end of the replicase polyprotein to generate two or three replicase products. Proteolytic processing of the coronavirus replicase polyprotein is essential for generating a functional replication complex. Therefore, the coronavirus replicase-encoded proteases, 3CLpro and PLpro, are potential targets for antiviral drug development.

P1551 | Recombinant SARS-CoV-2 Papain-like Protease DataSheet

Biomolecule/Target :

Synonyms: PLPro, PL-PRO, pp1a, Papain-like Protease, Replicase polyprotein 1a, ORF1a polyprotein, Plpro

Alternates names: PLPro, PL-PRO, pp1a, Papain-like Protease, Replicase polyprotein 1a, ORF1a polyprotein, Plpro

Taglines: Replicase polyprotein essential for generating a functional replication complex

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Coronavirus

Accession #: YP_009725299.1

Recombinant: FALSE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: < 1.0 EU/ug of the protein by LAL method.

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 38 kDa (6×His tag at the N-terminus)

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa Glu1564-Lys1878

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans