Relaxin-3, human recombinant | 7195

Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intra cerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

7195 | Relaxin-3 human recombinant DataSheet

Biomolecule/Target: Relaxin-3

Synonyms: H3 relaxin, Insulin-like peptide-7, INSL7

Alternates names: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen

Taglines: Binds specifically to the LGR7 receptor

NCBI Gene ID #: 355

NCBI Gene Symbol: FASL

Gene Source: Human

Accession #: P25445

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 g/ml in the presence of 2ng/ml of hFasL.

Biological activity: ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 g/ml in the presence of 2ng/ml of hFasL.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS