Resistin Antibody [RRES 07] | 36-014

Resistin Antibody [RRES 07] | 36-014 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Rat

Homology: N/A

Immunogen: Recombinant rat resistin.

Research Area: Obesity

Tested Application: E, WB

Application: ELISA: (direct or indirect: 1:5, 000-1:10, 000) . Western blot: (1:2, 000-1:10, 000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

Specificiy: Recognizes rat resistin. Detects a band of ~10kDa by Western blot. Does not cross-react with human and mouse resistin.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: RRES 07

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.