Retinoblastoma / Rb Antibody [SPM353] | 33-784

Retinoblastoma / Rb Antibody [SPM353] | 33-784 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse

Homology: N/A

Immunogen: A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.

Research Area: Cancer, Cell Cycle

Tested Application: WB, IP, IHC-P

Application: WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE) : 1-2 ug/ml for 30 min at RT
Titering of the Retinoblastoma antibody may be required for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: SPM353

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.