Rictor (Phospho-Thr1135)  polyclonal Antibody | BS65995

Rictor (Phospho-Thr1135)  polyclonal Antibody | BS65995 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IF IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background: Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rictor (Phospho-Thr1135) polyclonal Antibody detects endogenous levels of Rictor protein only when phosphorylated at Thr1135.

Molecular Weight: ~ 210 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Rapamycin-insensitive companion of mTOR; AVO3 homolog; hAVO3; RICTOR; KIAA1999

Immunogen: Synthetic phosphopeptide derived from human Rictor around the phosphorylation site of Threonine 1135.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Autophagy Signaling,