RIPK1 Antibody | 3291

RIPK1 Antibody | 3291 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (100%)

Immunogen: RIPK1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human RIP1.
The immunogen is located within amino acids 600 - 650 of RIPK1.

Research Area: Apoptosis

Tested Application: E, IHC-P

Application: RIPK1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 74 kDa

Validation: N/A

Isoform: N/A

Purification: RIPK1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: RIPK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RIP1 Antibody: RIP1 (Receptor Interacting Protein) , also known as RIPK1, is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell’s decision to live or die. RIP1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIP1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIP1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIP1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.