RNF111 Antibody (N-term) [APR04326G]

RNF111 Antibody (N-term) [APR04326G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RNF111 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-237 amino acids from the N-terminal region of human RNF111.

Cellular Localisation: Nucleus. Cytoplasm Nucleus, PML body {ECO:0000250|UniProtKB:Q99ML9}. Note=Upon TGF-beta treatment, translocates from nucleus to cytosol

Molecular Weight: 108862

Clone: Polyclonal

Gene Name: RNF111 (HGNC:17384)

Gene ID: 54778

Function: E3 ubiquitin-protein ligase (PubMed:26656854) . Required for mesoderm patterning during embryonic development (By similarity) . Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019, PubMed:16601693) . Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019, PubMed:16601693) . In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity) . Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (PubMed:17591695) . Associates with UBE2D2 as an E2 enzyme (PubMed:22411132) . Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23751493) . Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (PubMed:23751493) . Mediates ubiquitination and degradation of sumoylated PML (By similarity) . The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity) .

Summary: Tissue Location: Broadly expressed..

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: E3 ubiquitin-protein ligase Arkadia, 632-, RING finger protein 111, RNF111