RNF139 Antibody (C-Term) [ApR04942G]

RNF139 Antibody (C-Term) [ApR04942G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RNF139 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 604-636 amino acids from human RNF139.

Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein

Molecular Weight: 75994

Clone: Polyclonal

Gene Name: RNF139 (HGNC:17023)

Gene ID: 11236

Function: E3-ubiquitin ligase; acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death (PubMed:10500182, PubMed:12032852, PubMed:17016439) . Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER) (PubMed:19720873) . Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression (PubMed:19706601, PubMed:20068067) . Involved in the sterol-accelerated degradation of HMGCR (PubMed:22143767, PubMed:23223569) . This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane (PubMed:22143767) . In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR (PubMed:23223569) . The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed:22143767, PubMed:23223569) . Required for INSIG1 ubiquitination (PubMed:20068067) . May be required for EIF3 complex ubiquitination (PubMed:20068067) .

Summary: Tissue Location: Highly expressed in testis, placenta and adrenal gland. Moderate expression in heart, brain, liver, skeletal muscle and pancreas, and low expression in lung and kidney

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: E3 ubiquitin-protein ligase RNF139, 632-, RING finger protein 139, Translocation in renal carcinoma on chromosome 8 protein, RNF139 (HGNC:17023)