RSF1 Antibody (C-term) [AMM07672G]

RSF1 Antibody (C-term) [AMM07672G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RSF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1355-1389 amino acids from the C-terminal region of human RSF1.

Cellular Localisation: Nucleus Note=Localization is diffuse during mitosis (PubMed:12972596) . Co- localizes with SMARCA5 in the nucleus (PubMed:12972596)

Molecular Weight: 163821

Clone: Polyclonal

Gene Name: RSF1

Gene ID: 51773

Function: Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:12972596, PubMed:28801535) . Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596) . Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed:28801535) . The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed:28801535) . The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535) . Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed:11944984, PubMed:11788598) .

Summary: Tissue Location: Ubiquitously expressed. Highly expressed in the heart, skeletal muscle, kidney and placenta (PubMed:12972596) Expressed at low levels in the brain and colon (PubMed:12972596)

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex, RSF1, HBXAP, XAP8