Description
RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK2 has been shown to mediate growth factor signaling via RAS and MAPK leading to the induction of CREB serine-133 phosphorylation and activation of gene expression (1). Mutations in RSK2 have been shown to be responsible for Coffin-Lowry syndrome (CLS) which is a X-linked disorder characterized by severe psychomotor retardation, facial and digital dysmorphisms, and progressive skeletal deformations (2).
Biomolecule/Target: N/A
Synonyms: RSK, ribosomal protein S6 kinase, 90kDa, polypeptide 3
Alternates names: RSK, ribosomal protein S6 kinase, 90kDa, polypeptide 3, MAP kinase-activated protein kinase 1c
Taglines: Involved in signal transduction
NCBI Gene ID #: 4254
NCBI Gene Symbol: SCF
Gene Source: N/A
Accession #: P21583
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of RSK2 was determined to be 157 nmol /min/mg as per activity assay protocol.
Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of >5 x 10 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 112.0 kDa
Concentration: 0.1mg/ml
Appearance: Liquid
Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Reconstitution Instructions: Reconstitute the lyophilized human SCF in sterile HO to a concentration of 0.1-1 mg/ml. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A