RSK3, Active | 7774

RSK3 is a member of the RSK (ribosomal S6 kinase) family that encodes a 733-amino-acid protein with a unique N-terminal region containing a putative nuclear localization signal (1). RSK3 mRNA is widely expressed and is activated by growth factors, serum and phorbol ester. Upon stimulation, RSK3 translocates to the cell nucleus and phosphorylates nuclear proteins. RSK3 can bind to ERK1/2 and this association increases the duration of RSK3 activation (2).

7774 | RSK3 Active DataSheet

Biomolecule/Target: N/A

Synonyms: RSK, ribosomal protein S6 kinase, 90kDa, polypeptide 2

Alternates names: RSK, ribosomal protein S6 kinase, 90kDa, polypeptide 2

Taglines: Involved in signal transduction

NCBI Gene ID #: 17311

NCBI Gene Symbol: SCF

Gene Source: N/A

Accession #: P20826

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of RSK3 was determined to be 131 nmol /min/mg as per activity assay protocol.

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <10 ng/ml, corresponding to a specific activity of >1 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 112.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A