Rubicon Antibody | 7943

Rubicon Antibody | 7943 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
The immunogen is located within amino acids 240 - 290 of Rubicon.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 90, 101, 109 kDa
Observed: 91, 99 kDa

Validation: N/A

Isoform: N/A

Purification: Rubicon antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Rubicon antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Rubicon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1) . Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2) . Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2) .