Description
Rubicon Antibody | 7943 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
The immunogen is located within amino acids 240 - 290 of Rubicon.
Research Area: Autophagy
Tested Application: E, WB, IHC-P, IF
Application: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 90, 101, 109 kDa
Observed: 91, 99 kDa
Validation: N/A
Isoform: N/A
Purification: Rubicon antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Rubicon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1) . Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2) . Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2) .