Rubisco quantitation kit (Western blot) | AS09 409set

Rubisco quantitation kit (Western blot) | AS09 409set | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.

Host: Primary antibody: Rabbit, Secondary antibody: Goat,

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: N/A

Format: N/A

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 5000-1 : 10000 (WB)

Molecular weight: Rubisco protein standard: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Cynara cardunculus var altilis, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonana

Predicted Reactivity: Alpha proteobacteria, Algae (brown and red), Beta-proteobacteria, Dicots, Conifers, Cryptomonad, Cyanobacteria, Gamma-proeobacteria, Liverworts, Mosses, Prochlorophytes, PelwitschiaSpecies of your interest not listed? Contact us

Not reactive in: N/A

Additional Information: Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009) . Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.

Background: N/A

Reconstitution: N/A

Storage: RbcL antibody and protein standard: Please store at -20°C (6 months) or -80°C for long term storage (years) . Please avoid freezing and thawing of reconstituted antibodies, make aliquots instead. PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°C and for long term storage (1 year) at -20°C.

TAIR Nnumbre: N/A

Category: RUBISCO/Carbon metabolism

Research Area: Plant Biology, Photosynthesis