RXRA Antibody (Center) [AMR09792G]

RXRA Antibody (Center) [AMR09792G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RXRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-224 amino acids from the Central region of human RXRA.

Cellular Localisation: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:11915042, ECO:0000269|PubMed:12145331, ECO:0000269|PubMed:15509776, ECO:0000269|PubMed:17761950, ECO:0000269|PubMed:28167758}. Cytoplasm. Mitochondrion. Note=Localization to the nucleus is enhanced by vitamin D3 (PubMed:15509776) . Nuclear localization may be enhanced by the interaction with heterodimerization partner VDR (PubMed:12145331) . Translocation to the mitochondrion upon interaction with NR4A1 (PubMed:17761950, PubMed:15509776) . Increased nuclear localization upon pulsatile shear stress (PubMed:28167758)

Molecular Weight: 50811

Clone: Polyclonal

Gene Name: RXRA

Gene ID: 6256

Function: Receptor for retinoic acid that acts as a transcription factor (PubMed:11162439, PubMed:11915042) . Forms homo- or heterodimers with retinoic acid receptors (RARs) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes (PubMed:10195690, PubMed:11162439, PubMed:11915042, PubMed:28167758, PubMed:17761950, PubMed:16107141, PubMed:18800767, PubMed:19167885) . The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 to regulate transcription (PubMed:10195690, PubMed:11162439, PubMed:11915042, PubMed:17761950, PubMed:28167758) . The high affinity ligand for retinoid X receptors (RXRs) is 9-cis retinoic acid (PubMed:1310260) . In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression (PubMed:20215566) . On ligand binding, the corepressors dissociate from the receptors and coactivators are recruited leading to transcriptional activation (PubMed:20215566, PubMed:9267036) . Serves as a common heterodimeric partner for a number of nuclear receptors, such as RARA, RARB and PPARA (PubMed:10195690, PubMed:11915042, PubMed:28167758, PubMed:29021580) . The RXRA/RARB heterodimer can act as a transcriptional repressor or transcriptional activator, depending on the RARE DNA element context (PubMed:29021580) . The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes (PubMed:10195690) . Together with RARA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells (PubMed:28167758) . Acts as an enhancer of RARA binding to RARE DNA element (PubMed:28167758) . May facilitate the nuclear import of heterodimerization partners such as VDR and NR4A1 (PubMed:12145331, PubMed:15509776) . Promotes myelin debris phagocytosis and remyelination by macrophages (PubMed:26463675) . Plays a role in the attenuation of the innate immune system in response to viral infections, possibly by negatively regulating the transcription of antiviral genes such as type I IFN genes (PubMed:25417649) . Involved in the regulation of calcium signaling by repressing ITPR2 gene expression, thereby controlling cellular senescence (PubMed:30216632) .

Summary: Tissue Location: Expressed in lung fibroblasts (at protein level) (PubMed:30216632) . Expressed in monocytes (PubMed:26463675) . Highly expressed in liver, also found in kidney and brain (PubMed:24275569, PubMed:2159111, PubMed:14702039) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Retinoic acid receptor RXR-alpha, Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha, RXRA, NR2B1