S39A8 Antibody (Center) [AMM08679G]

S39A8 Antibody (Center) [AMM08679G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This S39A8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human S39A8.

Cellular Localisation: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Note=Localizes to the lysosome of activated T-cells (PubMed:19401385) . A large fraction of the protein is found intracellularly in microvascular capillary endothelial cells that constitute the blood-brain barrier (PubMed:31699897) . Localized and functional at both apical and basolateral membranes of microvascular capillary endothelial cells that constitute the blood-brain barrier (PubMed:31699897)

Molecular Weight: 49631

Clone: Polyclonal

Gene Name: SLC39A8 (HGNC:20862)

Gene ID: 64116

Function: Electroneutral transporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis or immunity (PubMed:12504855, PubMed:22898811, PubMed:23403290, PubMed:29337306, PubMed:26637978, PubMed:29453449) . Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation, a bicarbonate and a selenite anion or yet a metal cation and two bicarbonate anions (PubMed:27166256, PubMed:31699897) . May also transport iron, mercury and cobalt through membranes (PubMed:22898811) . Beside these endogenous cellular substrates, also imports cadmium a non-essential metal which is cytotoxic and carcinogenic (PubMed:27466201) . Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development (PubMed:29337306) . Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization (PubMed:29927450) . Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes (PubMed:23403290, PubMed:28056086) . Protects, for instance, cells from injury and death at the onset of inflammation (PubMed:18390834) . By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries (By similarity) . At the apical membrane of hepatocytes, reclaims manganese from the bile and regulates, through the systemic levels of the nutrient, the activity of manganese-dependent enzymes (PubMed:28481222) . Also participates in manganese reabsorption in the proximal tubule of the kidney (PubMed:26637978) . By mediating the extracellular uptake of manganese by cells of the blood-brain barrier, may also play a role in the transport of the micronutrient to the brain (PubMed:26637978, PubMed:31699897) . Through manganese cellular uptake also participates in mitochondrial proper function (PubMed:29453449) . Finally, also probably functions intracellularly, translocating zinc from lysosome to cytosol to indirectly enhance the expression of specific genes during TCR-mediated T cell activation (PubMed:19401385) .

Summary: Tissue Location: Ubiquitously expressed (PubMed:12504855, PubMed:22898811, PubMed:28056086, PubMed:31699897) . Expressed in thymus, placenta, lung, liver, pancreas, salivary gland and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas (PubMed:12504855) Expressed by macrophages (at protein level) (PubMed:28056086) Expressed by microvascular capillary endothelial cells that constitute the blood-brain barrier (at protein level) (PubMed:31699897)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: Zinc transporter ZIP8, BCG-induced integral membrane protein in monocyte clone 103 protein, LIV-1 subfamily of ZIP zinc transporter 6, LZT-Hs6, Solute carrier family 39 member 8, Zrt- and Irt-like protein 8, ZIP-8, SLC39A8, BIGM103, ZIP8