SAE2 Antibody | 5751

SAE2 Antibody | 5751 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (94%)

Immunogen: SAE2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SAE2.
The immunogen is located within amino acids 550 - 600 of SAE2.

Research Area: Homeostasis

Tested Application: E, WB, ICC, IF

Application: SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: SAE2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SAE2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SAE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SAE2 Antibody: ARX, SAE2, HRIHFB2115, UBLE1B, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SAE2 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.