SBP | Sedoheptulose-1, 7-bis phosphatase positive control/quantitation standard | AS15 2873S

SBP | Sedoheptulose-1, 7-bis phosphatase positive control/quantitation standard | AS15 2873S | Gentaur UK, US & Europe Distribution

Immunogen: N/A

Host: N/A

Conjugation: N/A

Clonality: N/A

Isotype: N/A

Purity: N/A

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: Primary antibodies matching SBPase standard are: AS15 2873 | Anti-SBPase | Sedoheptulose-1, 7-bis phosphatase, rabbit antibodies for SBPase quantification using quantitative western blot methodcollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: Standard curve: 3 loads are recommended (0.5, 2 and 4µl) .For most applications a sample load of 0.2 µg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Molecular weight: 27 kDa

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Background: SBPase (Sedoheptulose-1, 7-bis phosphatase) is a chloroplast enzyme involved in the carbon reduction of the Calvin cycle, part of carbohydrate metabolism.Source of SBPase standard: SBPase standard source is Sphingomonas wittichii strain RW1, overexpressed in E.coli bearing an N-terminal his6 tag.

Reconstitution: For reconstitution add 85 µl of sterile water, Please note that this product contains glycerol and might appear as liquid but is provided lyophilized

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Protein standards-quantitation

Research Area: Plant Biology, Photosynthesis