sCD34, Human Recombinant | P1286

CD34 is a highly glycosylated type I membrane protein that is selectively expressed on hematopoietic stem cells and vascular endothelium. It has been widely used as a molecular marker for the identification, isolation, and manipulation of hemopoietic stem cells and progenitors. CD34 can function as a regulator of hemopoietic cell adhesion by mediating the attachment of stem cells to bone marrow stromal cells or other bone marrow components. The full length human CD34 is a 385 amino acid protein, consisting of a 31 amino acid signal sequence, a 74 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain and a 259 amino acid extracellular domain. Recombinant Human sCD34 is a 258 amino acid polypeptide containing only the extracellular domain of the full length CD34 protein.

P1286 | sCD34 Human Recombinant DataSheet

Biomolecule/Target: sCD34

Synonyms: Soluble CD34, Hematopoietic progenitor cell antigen CD34, CD34

Alternates names: Growth/Differentiation Factor-11, BMP-11

Taglines: Molecular marker for hemopoietic stem cells and progenitors.

NCBI Gene ID #: 10220

NCBI Gene Symbol: GDF11

Gene Source: Human

Accession #: O95390

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.08-0.10 g/ml.

Biological activity: Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.08-0.10 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Keep pH below 5.0. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS