SESTRIN2 Antibody | 8023

SESTRIN2 Antibody | 8023 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (77%)

Immunogen: SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2.
The immunogen is located within amino acids 220 - 270 of SESTRIN2.

Research Area: Autophagy, Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 54 kDa
Observed: 53 kDa

Validation: N/A

Isoform: N/A

Purification: SESTRIN2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SESTRIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SESTRIN-2, SES2, SEST2, SESN2, HI95

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1) . SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2) . SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3) , thereby protecting cells against energetic stress-induced death (4) .