SFPQ Polyclonal Antibody | E-AB-62211

SFPQ Polyclonal Antibody | E-AB-62211 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: SFPQ, POMP100, PPP1R140, PSF

Application: WB, IHC, IF

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Epigenetics and Nuclear Signaling

Background: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant fusion protein of human SFPQ (NP_005057.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification Method: Affinity purification

Dilution: WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 72 kDa/76 kDa

Molecular Weight(Observed): 115 kDa