SGMS2 Antibody (C-term) [APR09908G]

SGMS2 Antibody (C-term) [APR09908G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SGMS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-365 amino acids from the C-terminal region of human SGMS2.

Cellular Localisation: Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Primarily localized at the plasma membrane with a small fraction at the Golgi apparatus.

Molecular Weight: 42280

Clone: Polyclonal

Gene Name: SGMS2 {ECO:0000303|PubMed:30779713, ECO:0000312|HGNC:HGNC:28395}

Gene ID: 166929

Function: Sphingomyelin synthase that primarily contributes to sphingomyelin synthesis and homeostasis at the plasma membrane. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction appears to depend on the levels of CER and DAG in the plasma membrane (PubMed:14685263, PubMed:17449912, PubMed:17982138, PubMed:18370930) . Does not use free phosphorylcholine or CDP-choline as donors (PubMed:14685263) . Can also transfer phosphoethanolamine head group of phosphatidylethanolamine (PE) on to ceramide (CER) to form ceramide phosphoethanolamine (CPE) (PubMed:19454763) . Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed:17449912, PubMed:17982138) . To a lesser extent, plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (PubMed:18370930, PubMed:21980337) . Required for normal bone matrix mineralization (PubMed:30779713) .

Summary: Tissue Location: Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2, SGMS2, SMS2