SGPP1 Antibody (N-term) [APR03792G]

SGPP1 Antibody (N-term) [APR03792G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SGPP1.

Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q9JI99}; Multi-pass membrane protein

Molecular Weight: 49108

Clone: Polyclonal

Gene Name: SGPP1 (HGNC:17720)

Gene ID: 81537

Function: Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid (PubMed:16782891) . Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (PubMed:12815058, PubMed:11756451, PubMed:16782891) . Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:11756451, PubMed:12815058, PubMed:16782891) . Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (By similarity) . Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic retiulum to the Golgi apparatus (PubMed:16782891) . The modulation of intracellular ceramide levels in turn regulates apoptosis (By similarity) . Via S1P levels, modulates resting tone, intracellular Ca (2+) and myogenic vasoconstriction in resistance arteries (PubMed:18583713) . Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:20798685, PubMed:18583713) . Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (By similarity) .

Summary: Tissue Location: Ubiquitous, with the strongest level in placenta and kidney.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P-Leica, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Sphingosine-1-phosphate phosphatase 1, SPPase1, Spp1, hSPP1, hSPPase1, 313-, Sphingosine-1-phosphatase 1, SGPP1