451 Recombinant Proteins and Cell culture
Sheep anti-Rabbit IgG (H&L), TRITC conjugated | AS10 1539
- SKU:
- 451-AS10 1539
- Availability:
- Usually ships in 5 working days
Description
Sheep anti-Rabbit IgG (H&L), TRITC conjugated | AS10 1539 | Gentaur UK, US & Europe Distribution
Immunogen: Purified rabbit IgG (H&L)
Host: Sheep
Conjugation: TRITC
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified sheep IgG.
Format: Lyophilized
Tested Application: N/A
Related Products: other fluorescence conjugated secondary antibodies against rabbit IgG (H&L)
Recommended Dilutions: The optimal working dilution should be determined by the investigator, Suggested starting dilution (s) : 1 : 20-1 : 2000 for most applications
Molecular weight: N/A
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L) . Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: FITC, TRITC
Research Area: Immunoassays