Sheep Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit | AE63367SH

Sheep Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit | AE63367SH | Gentaur UK, US & Europe Distribution

Species Reactivity: Sheep (Ovis aries)

Abbreviation: COL18A1

Alternative Name: FLJ27325; FLJ34914; KNO; KNO1; MGC74745; alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤6.7%

Inter-Assay: ≤6.3%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate COL18A1 in samples. An antibody specific for COL18A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL18A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL18A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL18A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Collagen, type XVIII, alpha 1, also known as COL18A1, alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains.The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .